Analysis of platelet-rich plasma extraction variations in platelet and blood components between 4 common commercial kits

Background: Platelet-rich plasma (PRP) has been extensively used as a treatment in tissue healing in tendinopathy, muscle injury, and osteoarthritis. However, there is variation in methods of extraction, and this produces different types of PRP. Purpose: To determine the composition of PRP obtained from 4 commercial separation kits, which would allow assessment of current classification systems used in cross-study comparisons. Study Design: Controlled laboratory study. Methods: Three normal adults each donated 181 mL of whole blood, some of which served as a control and the remainder of which was processed through 4 PRP separation kits: GPS III (Biomet Biologics), Smart-Prep2 (Harvest Terumo), Magellan (Arteriocyte Medical Systems), and ACP (Device Technologies). The resultant PRP was tested for platelet count, red blood cell count, and white blood cell count, including differential in a commercial pathology laboratory. Glucose and pH measurements were obtained from a blood gas autoanalyzer machine. Results: Three kits taking samples from the “buffy coat layer” were found to have greater concentrations of platelets (3-6 times baseline), while 1 kit taking samples from plasma was found to have platelet concentrations of only 1.5 times baseline. The same 3 kits produced an increased concentration of white blood cells (3-6 times baseline); these consisted of neutrophils, leukocytes, and monocytes. This represents high concentrations of platelets and white blood cells. A small drop in pH was thought to relate to the citrate used in the sample preparation. Interestingly, an unexpected increase in glucose concentrations, with 3 to 6 times greater than baseline levels, was found in all samples. Conclusion:This study reveals the variation of blood components, including platelets, red blood cells, leukocytes, pH, and glucose in PRP extractions. The high concentrations of cells are important, as the white blood cell count in PRP samples has frequently been ignored, being considered insignificant. The lack of standardization of PRP preparation for clinical use has contributed at least in part to the varying clinical efficacy in PRP use. Clinical Relevance: The variation of platelet and other blood component concentrations between commercial PRP kits may affect clinical treatment outcomes. There is a need for standardization of PRP for clinical use

Web Interactive Multimedia Technology Implementation: A University Context

Elluminate a web interactive multimedia technology (WIMT), was introduced in a large metropolitan university. The system incorporates several interactive and multimedia features, such as chat, audio, video, polling, screen-board and desktop sharing. This system provides real time collaboration when used in a university teaching and learning environment as it enables immediate feedback between participants across physical space. This provides opportunities for interactivity and relationship building between academics and students, a vital component of learning. However, there are also several constraints in using WIMT. This paper discusses the findings from a case study conducted using design science research approach employing semi-structured interviews to gather the implementer’s and adopter’s perspective on their use of WIMT. The findings demonstrate a practical approach used as well as the benefits and challenges in using WIMT for teaching and learning activities that can be used as a guide for others

Shade Effects on Athletic Field Playing Characteristics of Overseeded and Non-Overseeded Bermudagrass

In many athletic stadiums around the world, reduced light levels from the stadium structure can significantly reduce turfgrass quality and playing characteristics. In most warm-weather stadiums, the primary surface is bermudagrass which is commonly overseeded with perennial ryegrass to provide a winter and spring playing surface. The objective of this study was to examine the effects of four shade levels on turfgrass quality and playing characteristics of overseeded and non-overseeded bermudagrass (cv. Riviera). Shade levels included a non-shaded control and shade treatments which blocked 30%, 60%, and 90% of ambient light. These shade levels resulted in average daily PAR loads of 45.1, 29.6, 16.7, and 4.7 mol PAR / m2 / day, respectively. Data collection included both quality and playability characteristics. The effects of shading became obvious as the study continued into early summer, with the 90% shade plots losing almost all cover and the 60% shade plots losing up to 40% of coverage through mid-summer. The 30% shade plots had a slight decline in turfgrass coverage, although, still retained close to 90% coverage. Based on these results, a minimum daily light load of approximately 30 mol PAR / m2 / day is needed to sustain Riviera bermudagrass at or near 100% coverage. In the overseeded plots, the only shade treatment that caused a significant reduction in turfgrass coverage was the 90% shade treatment (4.7 mol PAR / m2 / day). There was a significant decrease in the amount of bermudagrass present in the overseeded plots in mid-summer, suggesting that increased shading was causing a shift in population from the warm-season bermudagrass to the cool-season perennial ryegrass. In both overseeded and non-overseeded plots, rotational resistance (traction) decreased even with modest levels of shade , which is likely correlated to a decrease in turfgrass densit

How sustainable household environment and knowledge of healthy practices relate to childhood morbidity in South Asia: analysis of survey data from Bangladesh, Nepal and Pakistan

Objectives Prevalence of diarrhoea and acute respiratory infection (ARI) is considerably high among South Asian children. The objective of this study is to compare the associations of sustainable household environment and knowledge of healthy practices with episodes of these diseases among the children in the region. Design The study analysed the latest, nationally representative and cross-sectional Demographic and Health Survey data. Setting Data from three countries were analysed: Bangladesh, Nepal and Pakistan. Participants Women aged between 12 and 49 years living in selected households provided information on 23 940 of their children under the age of 5 years. Primary outcomes measures The morbidity status of the children was recorded with respect to episodes of diarrhoea and/or ARI in the 2 weeks preceding data collection. Results Consuming unhygienic drinking water increased the risks of childhood diarrhoea, and use of solid fuel for indoor cooking increased the risk of ARI, across all three countries investigated. However, far more significant were the effects of mother's education, with incomplete primary education leading to an odds of diarrhoea approaching twice that of a mother with secondary education or higher (OR 1.70 in Bangladesh, 95% CI 1.16 to 2.49). Conclusions Results from the current research underline the importance of developing and implementing integrated strategic plans for mothers and children in the countries investigated. Promoting hygienic water and sanitation facilities can help reduce the prevalence of childhood diarrhoea. Replacing indoor solid fuel cooking arrangements with cleaner fuel or more airy conditions can help reduce the prevalence of ARI. However, these strategies need to be integrated with education for women to raise the likelihood that reduced risks are actually realised